Core facilities

The CHUM Research Centre is home to fifteen state-of-the-art core facilities and services. Our specialized equipment and the unique expertise of our staff allow us to offer personalized support that meets the research needs of researchers at the CHUM Research Centre as well as those of our industry- and university-based clients.

In order to facilitate interactions with clients and maximize the progress of research projects, CRCHUM also offers a platform, MéDIS, which offer targeted expertise in clinical research and metabolic diseases.

Animal facility

The CHUM Research Centre has an ultra-modern animal facility designed to accommodate several animal species.

The specialized accommodations include facilities for large species, rodents, fish and immunocompromised animals and allows researchers to conduct containment level 2 studies.

Our staff members are committed to maintaining the integrity of the animal model and to ensuring the accuracy of experimental results and the well-being of the animals. Specialized services include:

  • anaesthesia
  • surgery
  • maintaining rodent colonies
  • importing
  • quarantining rodents

We also offer support in the development of experimental protocols.

The CHUM Research Centre holds a Certificate of Good Animal Practice from the Canadian Council on Animal Care (CCAC).

All animal use must receive prior approval from the Institutional Animal Protection Committee (CIPA).

Our services are available to researchers from universities, hospitals and for projects in partnership with the industry.

SERVICE REQUEST

For any service or submission request, please fill out the electronic form.

Animal facility contact: animalerie.cr.chum@ssss.gouv.qc.ca.

Institutional Animal Protection Committee (CIPA) contact: cipa.chum@ssss.gouv.qc.ca.

FEES

+ Fee guide

Biobanks

The CHUM Research Centre is home to several biological material banks for research purposes (biobanks).

Several professionals are available to guide researchers who want to create a new bank or submit projects related to biobank samples.

Note that the use of the biobanks requires users to be aware of certain instructions.

SERVICE

  • Professional management of a secure and dedicated space for the storage of biobank samples
  • Implementation of new biobanks
  • Various storage temperatures: 22°C, 4°C, -20°C, -80°C and liquid nitrogen
  • Removal or addition of biological samples
  • Output management of paraffin blocks and blades for research projects
  • Repatriation of paraffin blocks and blades to a biobank
  • Migration of databases to the ATiM software

SERVICE REQUEST

For any service or submission request, please fill out the electronic form.

Supervisor: Cecilia Vargas
biobanque.cr.chum@ssss.gouv.qc.ca
514 890-8000, ext. 31439

Politique de la biobanque institutionnelle de recherche du CRCHUM du pavillon de Bullion à l’Hôpital Hôtel-Dieu (French only)

Procédure de gestion des accès, des équipements et du système d’alarme (French only)

Procédure d’utilisation du système d’alarme (French only)

+ Réglements relatif à la constitution, à la gestion et à l'utilisation des banques de recherche du CHUM (French only)

FEES

+ Fee guide

Biosafety Level 3 (BSL3)

The CHUM Research Centre's Biosafety level 3 (BSL3) core facility allows research teams to carry out studies on infectious diseases and safely handle certain biosafety level 3 pathogens.

Our facilities include eight cell culture rooms dedicated to different research programs. We also have a cytometry room for cell sorting.

Each room is equipped with two biological safety cabinets, incubators and work and storage spaces.

Our equipment and manipulation protocols comply with the requirements of the Public Health Agency of Canada and ensure user safety while preventing the spread of pathogens within the laboratories and the environment.

ÉQUIPEMENTS

  • 1 Cell sorting cytofluorometer FACS Aria IIIu
  • 18 Safety biological hood
  • 1 Ultracentrifuge
  • Sterilization
  • Long-term storage facility

Training and logistical support provided by platform supervisors.

Equipment guide

SERVICE REQUEST

For any service or submission request, please fill out the electronic form.

Supervisor: Olfa Debbeche, Ph.D.
Scientific advisor: Nicolas Chomont, Ph.D.

nc3.cr.chum@ssss.gouv.qc.ca
514 890-8000, ext. 35293

FEES

Available on request

Cellular imaging

The CHUM Research Centre cell imaging laboratory offers an array of equipment and comprehensive services:

  • state-of-the-art microscopes and equipment
  • microscopy and analysis services
  • microscopy training
  • support in planning and carrying out experiments.

The laboratory devotes a great deal of effort to the inspection and maintenance of the performance of its equipment, to the support provided to users and to the development of digital image analysis techniques.

Our services are available to researchers from universities, hospitals and for projects in partnership with the industry.

EQUIPMENTS AND SERVICES

Equipments

  • Upright Multi-Photon Confocal Microscope (Olympus, FV1000MPE/BK61WF)
  • Inverted Microscope - Biosafety Level 3 (Zeiss, AxioObserver Z1 Spinnig disk/TIRF)
  • Inverted Confocal Microscope (Leica Microsystems, TCS SP5)
  • Inverted Multi-Photon Confocal Microscope (Leica Microsystems, TCS SP5 MP)
  • Inverted Fluorescence Microscope  (Olympus, 1X71)
  • Widefield Microscope ( Leica Microsystems, DM4000B)
  • Image Analysis Workstation (Imaris 7.5.2 et ImagePro)

Services

  • Use of microscopes in self-service mode, after training (exception: the Olympus FV1000MPE confocal microscope must be used by core facility staff)
  • Turnkey service
  • Digital image analysis service
  • Development research assistance service
  • Tailor-made consultation and technical assistance for sample preparation, selection of the appropriate imaging technique, image acquisition and analysis
  • Training in microscopy, sample preparation, digital image acquisition and analysis

Equipment and services guide

SERVICE REQUEST

For any service or submission request, please fill out the electronic form.

Supervisor: Aurélie Cleret-Buhot, Ph.D.
Scientific advisor: Greg Fitzharris, Ph.D.

imagerie.cellulaire.cr.chum@ssss.gouv.qc.ca
514 890-8000, ext. 30948

FEES

+ Fee guide

Cellular physiology

The cellular physiology core facility specializes in the quantification of analytes (hormones, cytokines and other molecules) found in plasma/serum samples, by ELISA and AlphaLISA as well as by immunohistochemistry in tissues.

The core facility also has a Seahorse Analyzer to measure glycolytic flux and mitochondrial respiration, as well as a high content cellular imaging instrument (PerkinElmer Operetta) to quantify cellular phenotypic changes. The core facility can also, with the help of its industrial partners, develop customized ELISA, AlphaLISA and HTRF tests for new targets for screening or biomarker development.

EQUIPMENTS AND SERVICES

Equipments

  • Operetta cellular imager
  • Extracellular Flux Analyser
  • EnVision mutilabel plate reader
  • Nikon slide scanner
  • High definition Aperio slide scanner
  • Image analysis software Columbus
  • Calorimeter

Services

  • Immunoassay & biochemical determinations
  • Measurement of beta & alpha cell masses by immunohistochemistry
  • Seahorse XF24-3 flux analyser
  • Operetta high content imaging system

Equipment and services guide

SERVICE REQUEST

For any service or submission request, please fill out the electronic form.

Supervisor: Erik Joly, Ph.D.
Specialist: Mélanie Guèvremont, M.Sc.
Scientific advisor: Marc Prentki, Ph.D.

physio.cell.cr.chum@ssss.gouv.qc.ca
514 890-8000, ext. 23608

FEES

+ Fee guide

Citadel

The CHUM Center for the Integration and Analysis of Medical Data (CITADEL) is one of the initiatives adopted to facilitate a CHUM that learns, teaches and communicates. The project was born from the need to structure (and validate) data sources (clinical, administrative, financial, management, research) in order to use them for research and continuous improvement of health interventions.

The main objectives pursued by CITADEL:

  •  Integrate the information needed to improve patient care and increase the performance of the healthcare system,
  • provide easy, secure, appropriate and timely access to clinical administrative data to promote and facilitate research and innovation.

CITADEL's development is made possible thanks to the close collaboration of the Research Management Department, the Quality of care, Evaluation, Performance and Ethics Department, the Information Technology and Telecommunications Department and the Medical and University Affairs Department of the CHUM.

KEY FACTS

    +  10 integrated information systems
    +  17.9 million episodes of care
    +   3.6 million patient data


SERVICES

  • Methodological consultation
  • Preparation of chum clinico-administrative datasets for proposed analysis
  • Preparation of data sets of interest for proposed analysis
  • Data analysis and interpretation of results
  • Integration of new variables/data
  • Access and storage of research data – subject specific schema construction and maintenance for research groups
  • Prospective (live) data services available

SERVICE REQUEST

For information request or service request, please fill the request form (French only).

To know more about principles and measures to protect privacy and confidentiality as well as the general access terms, please consult CITADEL’s management framework document (French only).

For external academic projects or projects from the industry, please contact CITADEL’s team directly to know more about the usage terms and conditions.

FEES

+ To come

PERMISSIONS REQUIRED FOR DATA ACCESS REQUEST

Research projets, or any project within CITADEL's mandate, may be the subject of a data access request. The first step is to complete the service request form for a first examination of your project.

It is recommanded to contact the advisors of the CHUM's Office of Research Ethics at ethic.recherche.chum@ssss.gouv.qc.ca to verify the authorization required or wether your project should be rewiewed by the CER.

Contact Information

To contact CITADEL's team: citadel.cr.chum@ssss.gouv.qc.ca.

Team:

Dr. Michaël Chassé, M.D., PhD., FRCPC
Intensivist and Regular Researcher at the CHUM. Executive and Scientific Director of CITADEL

Kip Brown, MPAff
Research Associate at CRCHUM and Data Scientist. Director of Operations of CITADEL

François Harvey, B.Sc., DESS
Bioinformatician and Data Infrastructure specialist

Paule-Marjolaine Bodson-Clermont, M.Sc.
Biostatistician

Alina Dyachenko, M.Sc.
Biostatistician

Roy Nitulescu, B.Sc., MA
Biostatistician, Data Scientist

Rima Bouchakri, PhD
Data Scientist and Data Infrastructure specialist

Camille Craig, M.Sc.
Health Innovation and Evaluation Hub Coordinator

Marie-Noël Nadeau, B.A.A
Access Office Coordinator

Cytometry

The CHUM Research Centre's cytometry laboratory offers a complete range of services for multi-parameter cellular analysis.

We perform multi-parameter analyses of cells and other particles.

Done simultaneously, these analyses of several functional parameters, combined with surface markers, make it possible to identify and understand new mechanisms of cell immunity and viral pathogenesis.

We offer expert services in high-speed cell sorting for the purification of discrete primary cell subsets (peripheral blood or other tissues) or cell lines.

Our research centre is one of the few in Canada that offers a high-speed cell sorting service in a Biosafety Level 3 (BSL3) cabinet allowing the sorting of cells of patients infected with HIV and HVC.

Our services are available to researchers from universities, hospitals and for projects in partnership with the industry.

EQUIPMENTS AND SERVICES

Cytometers

  • Cytofluorometer FACS LSR II
    Cytometer 3 lasers (V405, B488, R633) 16 parameters, 14 colors 6V, 5B, 3R
  • Cytofluorometer FACS LSR II
    Cytometer 4 lasers (V405, B488, YG561, R633) 18 parameters, 16 colors 6V, 2B, 5YG, 3R
  • Cytofluorometer FACS LSR II
    Cytometer 5 lasers (UV355, V405, B488, YG561, R633) 20 parameters, 18 colors 3UV, 6V, 2B, 4YG, 3R
  • Cytofluorometer Fortessa
    Cytometer 5 lasers (UV355, V405, B488, YG561, R640) 20 parameters, 18 colors 3UV, 6V, 2B, 4YG, 3R
  • Cytofluorometer Symphony
    Cytometer 6 lasers (UV355, V405, B488, YG561, R640, IR 785) 31 parameters, 29 colors 7UV, 8V, 5B, 5YG, 3R, 1IR
  • Cytofluorometer FACS Aria IIIu Cell sorting
    Cytometer 4 lasers (V405, B488, YG561, R633) 17 parameters, 15 colors 5V, 2B, 5YG, 3R
  • Cytofluorometer FACS Aria IIIu Cell sorting (BSL3)
    Cytometer 4 lasers (V405, B488, YG561, R633) 17 parameters, 15 colors 5V, 2B, 5YG, 3R

Equipments and services guide

SERVICE REQUEST

For any service or submission request, please fill out the electronic form.

Supervisor: Dominique Gauchat, Ph.D.
Specialist : Philippe St-Onge, M.Sc.
Scientific advisor: Petronela Ancuta, Ph.D.

cytometrie.cr.chum@ssss.gouv.qc.ca
514 890-8000, ext. 35726

FEES

+ Fee guide

Experimental imaging

Combining biomedical engineering and medical expertise, the CHUM Research Centre's experimental imaging core facility offers high-tech medical imaging equipment and services dedicated entirely to human and animal research.

We also use the ultra-modern clinical research facilities and those of the animal core facility (surgery and shelter for animals).

Our equipment includes a 3 Tesla magnetic resonance imaging machine (MRI) and an ARTIS angiography system.

We also have a Cone Beam CT made by the company SiTech.

Our services are available to researchers from universities, hospitals and for projects in partnership with the industry.

EQUIPMENTS AND SERVICES

Equipment

  • Magnetic resonance imaging (MRI) 3 Tesla (Skyra 3T - Siemens)
  • Angiography (Artis Q - Siemens)
  • ConeBeam CT (NewTom 5G  - SiTech)
  • Fibroscan (KNS Canada)

Services

  • Magnetic resonance imaging
  • Angiography
  • Maxillo-facial imaging

+ Equipment and services guide

SERVICE REQUEST

For any service or submission request, please fill out the electronic form.

Supervisor: Jennifer Satterthwaite, M.Sc.
Scientific advisor: Jacques De Guise, ing., Ph.D.

imagerie.experimentale.cr.chum@ssss.gouv.qc.ca
514 890-8000, ext. 23483

FEES

+ Fee guide

Metabolomics

The metabolomics core facility is specialized in the quantification and analysis of key metabolites (<1,500 Daltons) and biomarkers for metabolic diseases by mass spectrometry (targeted metabolomics). Our team has developed several methods for the quantification of many water-soluble and lipid metabolites from biological samples such as of cells, biopsies, tissues, faeces and plasma. We continuously expand our service offer by developing methods for the quantification of new metabolites. Our technical services are modular and customers can participate at various steps of the analysis process to reduce costs (sample preparation, HPLC, results analysis, etc.).

Our services are available to researchers from universities, hospitals and for projects in partnership with the industry.

SERVICES

  • Water-soluble metabolites quantification
  • Lipidomics
  • HPLC

Equipments and services guide

SERVICE REQUEST

For any service or submission request, please fill out the electronic form.

Supervisor: Julien Lamontagne, Ph.D.
Specialist: Alexia Grangeon, M.Sc.
Scientific advisor:: Marc Prentki, Ph.D.

metabolomique.cr.chum@ssss.gouv.qc.ca
 

FEES

+ Fee guide

Molecular pathology

The CHUM Research Centre's molecular pathology laboratory offers several services that aim to support researchers with the histological and pathological aspects of their projects.

The laboratory offers a range of flexible services and specialized equipment making it possible to meet a variety of needs, including:

  • H&E staining
  • paraffin block or fresh tissue section
  • construction of tissue microarrays (TMAs)
  • immunohistochemical or immunofluorescent staining
  • slide digitization
  • image analyses

Our services are available to researchers from universities, hospitals and for projects in partnership with the industry.

EQUIPMENTS AND SERVICES

Equipments

  • Microtome (Leica Microsystems, RM2125)
  • Microscope (Olympus Optical, BX31TF)
  • Automated IHC slide staining system (Ventana/Roche, Benchmark)
  • Cryostat ( Leica Microsystems et CM1950110111)
  • Histology manual tissue arrayer (TMA) (Beecher, 4508-DM et Pathology Devices, TM Arrayer)
  • Slide scanner (Olympus Optical, VS110, BX61VSF)
  • H&E slide stainer (Shandon, Varistain XY)

Services

  • Paraffin embedding of tissues in blocks
  • Microtome sectioning
  • Microscopy
  • Cryostat sectioning
  • Automated IHC slide staining system
  • H&E coloration
  • Tissue microarray (TMA)
  • Cell pellet preparation
  • Sampling of tissues in paraffin blocks (punch)
  • Retrieval of blocks and slides
  • Automated image analysis
  • Access to a pathologist service (Dominique Trudel, M.D., Ph.D., FRCPC ; dominique.trudel.chum@ssss.gouv.qc.ca)

Equipment and services guide

SERVICE REQUEST

For any service or submission request, please fill out the electronic form.

Supervisor: Liliane Meunier, M.Sc. et Véronique Barrès, M.Sc.
Specialists: Gabriela Fragoso, Ph.D.
Scientific advisor: Anne-Marie Mes-Masson, Ph.D.

pathologie.moleculaire.cr.chum@ssss.gouv.qc.ca
514 890-8000, ext. 31296

FEES

+ Fee guide

PUBLICATIONS

+ Publication list

Pharmacokinetics

The core facility offers a range of services and a unique expertise in the mass spectrometric quantification of xenobiotics and in the determination of drug metabolism. Our instruments are at the cutting edge of technology and permit a variety of applications directly related to the drug discovery and development process, life sciences and preclinical and clinical research.  Our services are available to researchers from universities, hospitals and for projects in partnership with the industry.

EQUIPEMENTS AND SERVICES

Equipments

  • Mass spectrometer Q Exactive Plus
  • Mass spectrometer Quantiva
  • Mass spectrometer Quantum Ultra
    Manufacturer: Thermo Scientific

Services

  • Bioanalytical method development in biological fluids
  • Full or partial method validation
  • Analysis of preclinical and clinical samples
  • In vitro / in vivo metabolism

+ Equipment and services guide

SERVICE REQUEST

For any service or submission request, please fill out the electronic form.

Supervisor: Fleur Gaudette, M.Sc., Chemist
pharmacocinetique.cr.chum@ssss.gouv.qc.ca
514 890-8000, ext. 14798

FEES

+ Fee guide

Phenotyping of the rodents – cardiovascular

The expertise of this core facility makes it possible to offer multiple in vivo analysis services in rodents, particularly in the fields of cardiac imaging, telemetry and various types of surgeries such as the implantation of probes and mini-pumps. The platform is based on several advanced instruments and technologies such as a SkyScan micro-CT scanner, an ART Optix MX2 optical imaging system for live animal studies and an EchoMRI NMR system, in addition to the rooms and telemetry applications. Our services are available to researchers in academic institutions, hospitals and industrial environments.

Our services are available to researchers from universities, hospitals and for projects in partnership with the industry.

EQUIPMENTS AND SERVICES

Equipments

  • Skyscan 1176 (Bruker microCT)
  • ART Optix MX2 (Advanced Research Technologies)
  • EchoMRI Analyzer-700 (EchoMRI)
  • Telemetry (Data sciences International - PA-C10, PA-C40, PA-C50, ETA-F10)
  • Metabolic cages (Harvard Apparatus - 52-6715, 52-6731)

Services

  • Telemetry for systemic arterial pressure, heart rate, activity, temperature and  electrocardiogram (ECG)
  • Assessing body composition by non-invasive quantitative magnetic resonance (EchoMRI700)
  • Visualization of endogenous cellular and molecular fluorescence process (ART Optix MX2)
  • Measuring the total intake of food and water and collecting urine and faeces rodents (metabolic cages)
  • Surgery for rats and mice: implantation of telemetric sensors, model of ischemia / reperfusion UUO kidney, catheterixzation of the jugular vein for systemic medication injection and catheterization of the carotid artery.
  • Data and image analysis

Equipment and services guide

SERVICE REQUEST

For any service or submission request, please fill out the electronic form.

Supervisor: Junzheng Peng, M.D., Ph.D.
Scientific advisor: Julie L. Lavoie, Ph.D.

pheno.cardiovasculaire.cr.chum@ssss.gouv.qc.ca
514 890-8000, ext. 31416

FEES

+ Fee guide

Phenotyping of the rodents – metabolic

The core facility offers a range of services unique in Canada for the study of carbohydrate and energy metabolism in rodents such as routine tolerance tests (glucose, lipids, pyruvate, insulin) and metabolic cages. The platform also has exceptional expertise in performing hyperglycemic clamps as well as Hyperinsulinemic-euglycemic clamps, which are more informative than tolerance test.

Our expertise covers:

  • research projects carried out at the cellular or tissue level and on animals,
  • complete metabolic phenotyping of genetically modified rodents,
  • evaluation of the effect of pharmacological or molecular interventions on carbohydrate and/or energy metabolism in healthy rodent models or in obese or diabetic models.

All surgical procedures, metabolic tests, dosing and data analyses are carried out by our staff.

SERVICES

  • Isolation of pancreatic islets
  • Insulin secretion on cell lines, isolated pancreatic islets (static or dynamic in perifusion) and in situ pancreas
  • In vivo carbohydrate homeostasis
    • Glucose tolerance test
    • Insulin tolerance test
    • Assessment of insulin secretion by hyperglycaemic clamp
    • Assessment of insulin sensitivity by hyperinsulinemic euglycemic clamp
  • In vivo energy metabolism
    • Metabolic cages
  • Blood chemistry
  • Experimental models
    • Different models of obesity and / or diabetes
    • Systemic chronic perfusion model of glucose and / or lipids

+ Services guide

SERVICE REQUEST

For any service or submission request, please fill out the electronic form.

Supervisor: Khalil Bouyankdan, Ph.D.
Specialists : Mélanie Éthier et Grace Fergusson, Techniciennes en santé animale (TSA)
Scientific advisor: Thierry Alquier, Ph.D.

thierry.alquier@umontreal.ca
514 890-8000, ext. 23628

FEES

+ Fee guide

Public Patient Partnerships (PPP) in Research

Patient engagement in research

Traditionally, patient’s 1 contribution to research and related activities has been limited to the role of participants, for example in clinical trials. Such voluntary participation in research is an essential contribution to science. In this way, participants help researchers determine whether the treatment or intervention is working as intended. However, patients can become more active in research through meaningful collaboration with the research team, referred to as patient engagement/partnership. In this context, patients become partners in the project and can actively participate in governing, setting research priorities, developing research questions and even conducting parts of the research.

Why is patient partnership important for research?

This type of participation promotes relevant and useful research for the patients involved. Patient partners can also collaborate with the research team to disseminate the results and communicate them to various audiences as well as to policy or other decision-makers. They can apply the results in the context of health or the community environment.

The PPP core facility is a group of professionals from various fields of expertise such as medicine, ethics, continuing education, evaluation, project management and patient partners.


1  The word patient is used here as a general term that includes all people living with a health condition or limitation. This also includes caregivers, friends and family. Source IRSC http://www.cihr-irsc.gc.ca/f/48952.html#p.

SERVICES

  • Training curriculum that aims to teach researchers and patient partners about the basic concepts and steps to build an effective patient partnership relationship;
  • Strategic advice for the preparation of grant applications involving patient partners and for the preparation of patient-engagement/partnership strategies;
  • Support in budget planning in connection with the patient partnership;
  • Support for the recruitment of patient research partners;
  • Accompaniment and support of researchers in the implementation of the patient partnership in their research project;
  • Support for the evaluation of the patient partnership.

Our services are available to researchers in academic institutions, hospitals and industrial environment.

+ Service Guide

SERVICE REQUEST

For any request for a quote or service, please complete this electronic form.

If you have any questions about this form, please contact Catherine Purenne.

plateformeppp.chum@ssss.gouv.qc.ca

FEES

+ Fee guide

Radiochemistry and cyclotron

Our highly specialized team has the expertise to produce radiopharmaceuticals for Positron Emission Tomography (PET) imaging studies involving diagnosis, follow-up and better understanding of diseases. For instance, the fluorine-18 (18F) can be incorporated into a fatty acid derivative, 14(R,S)-[18F]fluoro-6-thia-heptadecanoic acid ([18F]FTHA), which accumulates in tissues with abnormal fatty acid metabolism, and thus can be useful in identifying disturbances in fat metabolism in specific tissues/ organs.

Our team works in the implementation of existing radiotracers for clinical research. We are also conducting several innovative research projects developing new promising radiolabeled molecules to improve the detection, to monitor treatment efficacy and to better understand the progression of diseases such as cancer, diabetes, neurodegenerative and cardiovascular diseases. This non-invasive imaging technology allows a fast and efficient translation of important findings from the fundamental research to the clinic via preclinical research.

The production of these radiotracers is performed in GMP environment. More importantly, automated modules in shielded hot cells, including two cells equipped with telemanipulator robotic arms enable the synthesis and manipulation of these radiotracers without any exposure of the Radiochemistry and Cyclotron personnel to radioactivity.  Moreover, the distribution of these radiotracers to the injection room, either at the CRCHUM animal facility or at the new CHUM nuclear medicine facility for injections to patients, is greatly facilitated by a pneumatic system, which quickly delivers the radiotracers in less than a minute.

Our services are available to researchers from universities, hospitals and for projects in partnership with the industry.

EQUIPMENTS AND SERVICES

The CRCHUM Radiochemistry and Cyclotron core-facility has an IBA cyclotron Cyclone® 18/9 MeV. This particle accelerator is used to produce PET radioisotopes, including Carbon-11, Fluorine-18 (via a small or a large target) and the Nitrogen-13.

We have the necessary expertise and the equipments to manufacture existing F-18 and C-11 radiolabeled radiopharmaceuticals, as well as a qualified team in research and development of new PET radiotracers.

Our laboratory also offers several quality control tests, including endotoxin evaluation, residual solvent quantification by using gas chromatography (GC) and the chemical purity assessment of samples by using high pressure liquid chromatography (HPLC).

CYCLOTRON 18 MEV

  • 2 GMP laboratories
  • COMECER® shielded hot cells.
  • Chemistry laboratory
  • Quality control laboratory
  • IBA Synthera® Chemistry (F-18)
  • IBA Synthera® HPLC
  • 2 Synthra® RNplus Research (F-18 modules)
  • Synthra® MeIplus (C-11 module)
  • Agilent® gas chromatography (GC) with liquid or headspace auto-injector
  • Agilent® high pressure liquid chromatography (HPLC) with auto-injector
  • 2 HPLC Waters systems
  • HPLC Shimadzu system
  • Endosafe PTS Endotoxin system

Our cyclotron could also generate (15O2; 124I et 64Cu). 

SERVICE REQUEST

For any service or submission request, please fill out the electronic form or contact the Technical Supervisor.

Scientific Manager: Jean DaSilva, Ph.D.
Supervisor: Mehdi Boudjemeline, M.Sc.
Specialists : Alejandro Hernandez M.Sc.,  Charles Olivier Normandeau M.Sc.,  José-Mathieu Simard B.Sc.
Cyclotron engineer: Nabil Naim, Ing.

mehdi.boudjemeline.chum@ssss.gouv.qc.ca
514 890-8000, ext. 30589

FEES

For the reason of the unique features of each project in radiochemistry, a quotation for the entire project can be provided upon request.

Scientific Writing

In his famous book "How to write and publish a scientific paper", Robert Day wrote "A scientific experiment is not complete until the results have been published". In fact, publishing has become the cornerstone of science and an essential prerequisite for obtaining public/private funding to sustain in the scientific community. Writing scientific articles and funding applications is therefore an integral and essential part of the practice of science. Good writing skills have become an essential tool to, for example, explain or market through the publications the quality and scope of experimental results, and clearly express the newly generated ideas and concepts to try and get necessary funding to continue the process. Thus, having a good project idea only fulfills half of the grants funding application’s requirements, and the other half is how you will sell the idea and convince the funding agency to support you. In summary, you should pay close attention to the format and content of your documents.

Grant and paper writing take time and considerable effort, and often enough investigators feel frustrated thinking they could do a better use of their time thinking about new ideas or analyzing data. With several years of experience in writing successful fund applications with industry and public funding bodies, and writing several publications in prestigious scientific journals, the Scientific Writing core facility can help you better organize and present your results, ideas and concepts in order to express your knowledge and convince your peers in an optimal way

SERVICES

  • Correcting/ improving grant applications and manuscripts
  • Direct grant/ manuscript writing help
  • Giving ideas to include translational approaches
  • Identify any weak points and to provide potential solutions
  • Student fellowship applications
  • Industry-related & drug discovery projects/ patents

Levels of service

      A. Only editing to identify weak points.
      B. Editing to improve presentation, grammar and syntax.
      C. More extensive editing, and suggestions to improve content (ex. translational approach).
      D. Help to write new documents.

(Time needed depends on the requested service)

SERVICE REQUEST

Pour toute demande de soumission ou de service, veuillez remplir ce formulaire électronique.

Supervisor: S.R.Murthy Madiraju, Ph.D.
murthy.madiraju@crchum.qc.ca
514 890-8000, ext. 23610

FEES

+ Fee guide

Transgenesis and animal modelling

The CHUM Research Centre's transgenesis and animal modelling core facility allows researchers to create genetically modified animals.

Our team offers researchers the support needed to establish and maintain their animal colonies and is available for consultation on matters related to genetically modified mouse and rat colonies.   

Our services are available to researchers from universities, hospitals and for projects in partnership with the industry.

All animal use must receive prior approval by the Institutional Animal Protection Committee (CIPA).

SERVICES

  • Pronuclear microinjection in mice and rats
  • Mouse sperm cryopreservation
  • Microinjection of embryonic stem cells
  • Electroporation of ES cells
  • Rodent genotyping
  • Chromosome count
  • Embryo cryopreservation
  • Embryo thawing
  • Embryo transfer

Services guide

+ Cryopreservation Annex

+ Cryopreservation form

+ Cryopreservation and rederivation form

+ Guide testing for CRISPRCas9 form

+ Rodent model generation by ES microinjection form

+ Rodent model generation by pronuclear microinjection form

SERVICE REQUEST

 

For any service or submission request, please fill out the electronic form.

Supervisor:  Jean-Francois Schmouth, Ph.D.
Specialists : Mariette Ouellette, Guillaume Bernas, Andrea Barrios
Scientific advisor: Christine Vande Velde, Ph.D.

animalerie.tg.cr.chum@ssss.gouv.qc.ca
514 890-8000, ext. 35224

FEES

+ Fee guide

Unit for Innovative therapies (UIT)

The CRCHUM's therapeutic unit is a clinical core facility that allows researchers and their teams to conduct research projects requiring a surveillance of participants ranging from a few hours to several days.

This facility includes:

  • 4 beds placed in isolated rooms with individual bathrooms;
  • 11 stretchers placed in separate cubicles.

Each room/cubicle is equipped with a multifunctional computer station that provides access to the patient's electronic medical record and any other administrative research or clinical application.

The beds and stretchers are oriented in a way that allows them to be seen from one of the 3 nursing stations, thus ensuring continuous supervision of the participants.

Each nursing station is equipped with a telephone and 2 computers with monitors and printers as well as a secure space to store confidential documents.

The unit is also equipped with a biosafety level 1 laboratory with a refrigerated centrifuge and biological safety cabinet.

Freezers at -20°C and freezers at -80°C are available on the same floor to store samples and specimens.

Our services are available to researchers in academic institutions, hospitals and for projects conducted in partnership with industries.

EQUIPMENT

In addition to standard medical supplies, the therapeutic unit has currently the following equipment:

  • 1 automated pharmacy cabinet;
  • 2 care carts;
  • 2 blood collection trolleys;
  • 3 portable oto-ophthalmoscopes;
  • 5 portable electrocardiograms;
  • 2 bathroom scales;
  • 8 telemetry systems connected to the hospital's intensive care unit;
  • cardiorespiratory monitors linked to the hospital's intensive care unit;
  • 6 multiparameter monitors for heart rate, blood pressure, oxygen saturation and temperature;
  • 11 electric syringe pumps;
  • 19 positive displacement pumps (9 triple and 10 single);
  • 1 glucometer (linked to the patient's electronic medical record);
  • 4 synchronized clocks.

Training and logistical support will be provided by the core facility staff.

Our equipment meets the standards required in clinical research.

SERVICE REQUEST

For internal customers, please complete the form available on the Intranet.

For external customers, contact the technical manager.

Xavier Levac PhD, MBA, MPDP, RN
xavier.levac.chum@ssss.gouv.qc.ca
514 890-8000, ext. 13727

FEES

Rates available on request.


 


Policies and procedures

To come