Large-scale cell analysis: conduct your investigations with the help of the Operetta CLS

The CRCHUM Cellular physiology core facility now has cutting-edge high-throughput imaging equipment available for use: the Operetta CLS, designed by Revvity, previously named PerkinElmer.

This confocal cell imager allows for advanced phenotypic analyses of cultured cells, spheroids and organoids. Whether for basic science or translational research projects, the Operetta CLS provides a powerful and adaptable solution to meet your large-scale cell analysis needs.

Unlike conventional microscopy on slides, the Operetta CLS uses microplates (96 to 1,536 wells), allowing for simultaneous screening of dozens, if not hundreds, of experimental conditions. It’s especially well designed for projects involving screening chemical compounds, small interfering RNAs or antibodies.

Exploring cell complexity with precision and depth

Due to its automatic confocal imaging system, the Operetta CLS can:

• Capture high-resolution images of complex cell structures
• Produce 3D reconstructions of tumoural spheroids and organoids
• Provide in-depth analyses of cell proliferation, migration and apoptosis
• Track the cell differentiation and variations in volume or in organelle mass (e.g., mitochondria)
• Quantify changes in localization or in the level of intracellular protein

Its capacity to detect several fluorescent markers simultaneously facilitates the study of complex phenotypes and of cell sub-populations within primary cultures or 3D models. You can also track the dynamic evolution of cells in environments that closely mimic human tissues.

A must-have tool

Combining powerful analytics, automatization and experimental flexibility, the Operetta CLS is a strategic tool to accelerate immunology, virology, oncology, neuroscience research… And much more.

Come discover the potential of multiparameter cellular imaging at the cellular physiology core facility. Our specialists will guide you through each major step: planning, set-up and analysis.

Author: Erik Joly, Core Facilities’ Echo committee