Cellular imaging

Manufacturer: Olympus
Model: FV1000MPE/BK61WF

Self-service: no
With service: yes

Upright point scanner confocal microscope with multi-photon laser and incubation chamber.
The system is equipped with 3 regular PMTs and 2 non-descanned detectors (NDD) for multi-photon.
The multiphoton laser is a tunable Ti:Saf laser 800-1 300 nm.
The motorized stage of the microscope can be removed to put a fixed stage adjustable in height for intra-vital imaging.

Objectives available
4X/0.16, 10X/0.4, 20X/0.75, 40X/0.95, 63X/1.35Oil, 20X/1.00 Water Dipping lens and 60X/1.2 Water Dipping lens

Excitation laser lines available
405, 458, 488, 515, 543 and 635 nm

Particularity
System with a black XL-Unit incubation chamber; the temperature can be controlled for intra-vital imaging studies.

Applications
ZStacks, 2D and 3D projections, FRET, FRAP, colocalization, sequential multi-dimensional acquisitions, tiling, multi-position acquisitions and spectral imaging.
The microscope has a multiphoton laser allowing imaging of thick samples and that can be configured for intravital imaging.

Manufacturer: Zeiss
Model: AxioObserver Z1 Spinning disk/TIRF

Self-service: yes
With service: yes

Spinning-disc inverted confocal microscope with TIRF module, small incubation chamber, Definite Focus module and Piezo Z-Focus Drive.
The system is equipped with a Yokogawa CSU-X1 spinning-disc linked to a highly sensitive EMCCD Evolve 512 Photometrics camera.
The motorized stage can accommodate a small incubation chamber with temperature and CO₂ control from Tokaï Hit.
The system is also equipped with a lens heater.
The system is associated to a synchronised unit to do ultra-fats Z stacks with a Piezo Z-focus Drive.

Objectives available
10X/0.3, 40X/1.3Oil DICIII and 100X/1.46Oil DICIII

Excitation laser lines available
405, 488, 561 and 639 nm

Particularity
This microscope is especially equipped for fast live imaging. It’s located in the containment level 3 area, so it can be used to image infected cells by viruses such as HIV or hepatitis.

Applications
Widefield microscopy, Optical sectioning, TIRF, live cell imaging (short and long term), Zstacks, 2D/3D projections, DIC.

Manufacturer: Leica Microsystems
Model: TCS SP5

Self-service: yes
With service: yes

Inverted point scanner confocal microscope with 3 spectral PMTs and a TLD module for brightfield or Ph acquisitions.
With this microscope, all wavelengths from 300 to 800 nm can be detected, with a precision of 3 nm and a 5 nm minimal band width.
It’s of great interest when the spectral resolution is critical.

Objectives available
10X/0.3, 20X/0.5, 40X/1.4Oil, 63X/1.4Oil and 100X/1.4Oil

Excitation laser lines available
458, 476, 488, 514, 561 and 633 nm

Applications
Optical sectioning, ZStacks, 2D and 3D projections, lambda stacks, spectral deconvolution, FRET, FRAP, colocalization, sequential multi-dimensional acquisitions.

Manufacturer: Leica Microsystems
Model: TCS SP5 MP

Self-service: yes
With service: yes

Inverted point resonant scanner confocal microscope with multiphoton laser, Z-galvo and incubation chamber.
The system has 3 spectral PMTs, a TLD module for brightfield, Ph or DIC acquisitions, 2 non-descanned detectors (NDDs) for multiphoton mode and a 8kHz resonant scanner for fast live imaging.
The multiphoton laser is a Ti:Saf 800-1 300 tunable.
With this microscope, all wavelengths from 300 to 800 nm can be detected, with a precision of 3 nm and a 5 nm minimal band width.
It’s of great interest when the spectral resolution is critical.

Objectives available
10X/0.3, 20X/0.7 Imm (Oil,Glyc), 63X/1.4Oil, 63X/1.4Glycerol and 100X/1.4Oil

The system is equipped with a motorized encoded stage.
The Z-Gavo allows precize and fast stack imaging.

Excitation laser lines available
405, 458, 476, 488, 514, 561 and 633 nm

Particularity
XL-Unit incubation chamber with temperature and CO₂ controlled.

Applications
Optical sectionning, ZStacks, 2D and 3D projections, lambda stacks, spectral deconvolution, FRET, FRAP, colocalization, sequential multi-dimensional acquisitions, tiling and multi-positionning.
Fast imaging for live video-microscopy (resonant scanner and Z-Galvo).
The multiphoton mode can achieve deep samples.

Manufacturer: Olympus
Model: IX71

Self-service: yes
With service: yes

Widefield inverted microscope for brightfield and/or fluorescence acquisitions on slides, multiwell plates or dishes.

Objectives available
10X/0.4, 20X/0.75, 40X/0.95 and 60X/1.3Water.

3 filter cubes available: blue (DAPI, Hoesch, Alexa405), green (GFP, FITC, Alexa488) and red (TxRed, mCherry, Alexa594).

Applications
Imaging in 3 colors on petri dishes or plates. The camera is a dual camera and can acquire in monochrome mode or color mode.

Manufacturer: Leica Microsystems
Model: DM4000B

Self-service: yes
With service: yes

Upright widefield microscope for transmitted light acquisition on slides.

TL modes available
BF and PH

Objectives available
Fluotar 10X/0.3, 20X/0.5, 40X/0.75 and 100X/1.3Oil

Applications
Transmitted light acquisition (BF or Ph), white balance and gamma adjustable with the software.

Manufacturer: Bitplane
Model: Imaris 7.5.2

Self-service: yes
With service: yes

Standard Imaris
Imaris MeasurmentPro
Imaris InPress.

Interactive processing and visualization software for 3D and 4D microscopic images.
Operates on 8, 16 and 32 bit multi-channel images. Includes slicer, sections, gallery, shadow projection ray tracer, realtime volume visualization.
Automatic generation of isosurfaces from a 3D image by thresholding. Identification of individual closed surfaces as objects (counting and grouping features).
Ideal for quantification of labeled components.
Simultaneous visualization of surfaces created from one or multiple channels with sections or volume rendered 3D or 4D data.
Powerful scene editor with multiple clipping planes, multiple light sources, calibration grid, and advanced surface property editor.
Specific readers for Biorad, DeltaVision, ICS/IDS, Leica, Olympus, Universal Imaging, Tiff series, TILLVisION, OME, and Zeiss image formats.
Interactive distance measurement in 3D on OrthoSlicers and Surfaces.
Statistical analysis of voxels enclosed by isosurface objects.
4D extension for interactive image processing and visualization of time series.
Time slider in views allow real-time inspection of 4D data sets even larger than RAM.
Automatic movie generation. Text and statistics annotation in 2D, 3D, or 4D.
Users interactively attach annotations to a 3D location in either the 3D view or in any of the 2D views.
Choices for color, transparency, fonts and styles are available for the text, text box, and pointer.
InPress exports to html.

Manufacturer: MediaCybernetics
Model: ImageProPlus

Self-service: yes
With service: yes

2D and 3D deconvolution, 3D analysis, cell counting, etc.