Phenotyping of the rodents – metabolic

The core facility offers a range of services unique in Canada for the study of carbohydrate and energy metabolism in rodents such as routine tolerance tests (glucose, lipids, pyruvate, insulin) and metabolic cages. The platform also has exceptional expertise in performing hyperglycemic clamps as well as Hyperinsulinemic-euglycemic clamps, which are more informative than tolerance test.

Our expertise covers:

  • research projects carried out at the cellular or tissue level and on animals,
  • complete metabolic phenotyping of genetically modified rodents,
  • evaluation of the effect of pharmacological or molecular interventions on carbohydrate and/or energy metabolism in healthy rodent models or in obese or diabetic models.

All surgical procedures, metabolic tests, dosing and data analyses are carried out by our staff.
 

SERVICES

  • Isolation of pancreatic islets
  • Insulin secretion on cell lines, isolated pancreatic islets (static or dynamic in perifusion) and in situ pancreas
  • In vivo carbohydrate homeostasis
    • Glucose tolerance test
    • Insulin tolerance test
    • Assessment of insulin secretion by hyperglycaemic clamp
    • Assessment of insulin sensitivity by hyperinsulinemic euglycemic clamp
  • In vivo energy metabolism
    • Metabolic cages
  • Blood chemistry
  • Experimental models
    • Different models of obesity and / or diabetes
    • Systemic chronic perfusion model of glucose and / or lipids

+ Services guide
 

SERVICE REQUEST

For any service or submission request, please fill out the electronic form.

Supervisor: Khalil Bouyankdan, Ph.D.
Specialists : Mélanie Éthier et Grace Fergusson, Techniciennes en santé animale (TSA)
Scientific advisor: Thierry Alquier, Ph.D.

thierry.alquier@umontreal.ca
514 890-8000, ext. 23628
 

FEES

+ Fees 2022-2023

 

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