Cellular physiology

List of services

• Immunoassays and biochemical assays for rodent and human samples

  Quantification of hormones, cytokines and peptides present in blood (plasma and serum) and in incubation media by ELISA, TR-FRET and AlphaLISA and by the use of highly sensitive microplate readers. 

  Examples of analytes: insulin, C-peptide, glucagon, CRP, cortisol, TNF-alpha, testosterone, leptin, Il-6. 

  Biochemical assays in humans and rodents (miniaturized assays). 

  Examples of analytes: triglycerides, fatty acids, glucose, fructose, cholesterol, creatinine, urea. 

  Development of custom assays for proteins and other targets of interest.

  
• Morphometry and quantification of cell masses

  Morphometry service (sizes, cell distribution) for adipocytes, islets of Langerhans and other cell types.

  Quantification of the relative cell masses present in a tissue or cell proliferation (Ki-67) by immunohistochemistry, using a high definition slide scanner.

  Exemples of results

  

  Measurement of beta and alpha cell masses of the islet of Langerhans.
  Immunohistochemistry of pancreatic sections in normal mice. Double staining: insulin (red) and glucagon (brown). Hematoxylin counterstain of Harris.

  

  Measurement of islet of Langerhans beta cell proliferation.
  Immunohistochemistry of pancreatic sections in diabetic rodent models. Double staining: insulin (red) and Ki-67 (brown). Harris hematoxylin counterstain.

   

  

  Segmentation of adipocytes

  

  Measurement of beta cell mass.
  Immunohistochemistry of pancreatic sections in normal mice. Simple labeling for insulin (red). Harris hematoxylin counterstain.

   

• Phenotypic analyzes

  Use of a PerkinElmer Operetta high-throughput cell imager for phenotypic analyzes in microplates (96 or 384 wells). 

  The Operetta analyzes the cell phenotype from endogenous fluorescence (autofluorescence) or through the use of specific fluorescent markers.  It can be used in confocal mode or not.   

  Examples of application: rapid and highly sensitive measurement of apoptosis, proliferation, cell migration, intracellular protein translocations, as well as mitochondrial mass and activities. 

  The Operetta is available for self-service use. 

  Contact the platform to be trained or to learn more about the device.