Cellular physiology

Liste des équipements

• PerkinElmer Operetta CLS High Throughput Analysis System

  The Operetta is a high throughput (96 or 384 well) microplate imager for high content analysis (HCA). It can acquire, analyze and manage fluorescence images by combining high power LED excitation (4 LEDs) and a large format sCMOS camera

  Learn more about PerkinElmer's Operetta CLS

   

  The software that controls the instrument (Harmony) offers reliable and fast discrimination (confocal mode or not) of phenotypes, even in complex cellular models (e.g.: organoids). Temperature and CO2 control, as well as water immersion objectives, make this system ideal for live cell imaging. 

  Contact the core facility for more details or to be trained to use the device yourself: physio.cell.cr.chum@ssss.gouv.qc.ca

  The following documents present examples of applications:

  Analyzing Chemokinesis of Cancer Cells

  Cytotoxicity Studies on Live Primary Human Hepatocytes

  Analysis of Stem Cell Differentiation

  Studying NF-kB Signaling Dynamics

  Screening HDAC Inhibitors

  
• PerkinElmer EnVision 2104 Multifunction Microplate Reader

  This microplate reader is fast, sensitive and very versatile. It offers optimized performance for each application: luminescence, fluorescence and absorbance.

  The reader is equipped with a high energy flash lamp and high-speed detectors to accommodate a wide range of assays including cellular, GPCR, reporter gene, enzyme, kinase, ELISA, AlphaLISA and TR-FRET. The superior detection sensitivity of this microplate reader comes from the specific optical mirror modules for each application. The device also has injectors for, for example, calcium influx measurement tests.

  PerkinElmer 2104 EnVision – Specifications

  PerkinElmer 2104 EnVision – Instruction Manual

   

  Contact the core facility for more details: physio.cell.cr.chum@ssss.gouv.qc.ca

  

   

• Biochrom ASYS UVM 340 microplate reader

  The UVM 340 has a monochromator that can accommodate absorbance measurements over a very wide range of wavelengths. A useful feature of the device is that it allows scanning of a single well on the plate for a wavelength range of 340-800 nm. The kinetics of a reaction can also be measured.

  Biochrom Asys UVM 340

   

  Contact the core facility for more details: physio.cell.cr.chum@ssss.gouv.qc.ca

• Nikon CoolScan 9000ED Slide Scanner

  This high-speed, multi-sample digitizer can achieve true optical resolution of 4000 DPI to digitize histological slides. It is mainly used to quantify cell types from a tissue such as the mass of pancreatic beta cells.

  Nikon CoolScan 9000ED – Manuel de l'utilisateur

   

  Contact the core facility for more details:  physio.cell.cr.chum@ssss.gouv.qc.ca

  

   

• Leica Aperio ScanScope CS High Resolution Slide Scanner

  The Aperio CS is a high-resolution slide scanner featuring a five-slide load capacity and 20x and 40x magnifications. 

  The device is accompanied by powerful analysis software including several algorithms (e.g. counting of positive pixels and rare events) and the eSlide Manager to manage image libraries. The device is used for morphometry analyzes (distribution and frequency of cell dimensions), for example for islets of Langerhans and adipose tissue, but it can be used for several other applications.

  Aperio ScanScope CS – Specifications

   

  Contact the core facility for more details: physio.cell.cr.chum@ssss.gouv.qc.ca