Just over a year ago, the Molecular Pathology core facility acquired the Lunaphore Comet, thanks to a generous donation from the CHUM Foundation. This cyclic immunofluorescence device can run up to 40 markers on a single slide.
With the help of a CRCHUM research and development program, we were able to build two tissue microarrays (TMAs) to validate a large panel of antibodies on human and mouse tissues.
These two TMAs—one based on human tissue and the other on mouse tissue—are available to the CRCHUM’s scientific community to validate their antibodies.
Several tissue options are available: imaging a 12.5 x 12.5 mm area on a slide of FFPE tissue or fresh tissue (whole tissue or tissue micropatterning).
Once Comet labelling has been completed, the Molecular Pathology core facility can take your slide and apply H&E staining or traditional IHC, which can then be aligned with the image using the phenoplex module and the IA module of the VIS software.
The Fondation du CHUM supported the core facility with the purchase of the phenoplex module, while the Institut du cancer de Montréal provided financial assistance for the acquisition of the AI module.
Authors: Véronique Barrès and Liliane Meunier, Core Facilities’ Echo committee
Any questions?
Contact the Molecular Pathology core facility team
How can the Lunaphore Comet be used to validate antibodies?
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The Core Facilities’ Echo