The CHUM Research Centre's molecular pathology laboratory offers several services that aim to support researchers with the histological and pathological aspects of their projects.
The laboratory offers a range of flexible services and specialized equipment making it possible to meet a variety of needs, including:
- H&E staining
- Paraffin block or fresh tissue section
- Construction of tissue microarrays (TMAs)
- DNA and RNA extraction as well as sample quality control
- Immunohistochemical or immunofluorescent staining
- Slide digitization
- Image analyses
Our services are available to researchers from universities, hospitals and for projects in partnership with the industry.
EQUIPMENTS AND SERVICES
Equipments
- Microtome (Leica Microsystems, RM2125)
- Microscope (Olympus Optical, BX31TF)
- Automated IHC slide staining system (Ventana/Roche, Benchmark)
- Cryostat ( Leica Microsystems et CM1950110111)
- Histology manual tissue arrayer (TMA) (Beecher, 4508-DM et Pathology Devices, TM Arrayer)
- Microscope slide scanner (Leica, Aperio Versa 200)
- H&E slide stainer (Shandon, Varistain XY)
- Qiacube (Qiagen)
- Tape Station (Agilent, 4150)
Services
- Paraffin embedding of tissues in blocks
- Microtome sectioning
- DNA and RNA extraction as well as sample quality control
- Microscopy
- Cryostat sectioning
- Automated IHC slide staining system
- H&E coloration
- Tissue microarray (TMA)
- Cell pellet preparation
- Sampling of tissues in paraffin blocks (punch)
- Retrieval of blocks and slides
- Automated image analysis
- Investigative histopathology (Feryel Azzi ;
feryel.azzi.chum@ssss.gouv.qc.ca)
+ Equipment and services guide
SERVICE REQUEST
For any service or submission request, please fill out the electronic form.
Supervisor: Liliane Meunier, M.Sc. et Véronique Barrès, M.Sc.
Specialists: Isabelle Clément, M.Sc. et Feryel Azzi, Investigative histopathology - PHQ ITMT
Scientific advisor: Anne-Marie Mes-Masson, Ph.D.
pathologie.moleculaire.cr.chum@ssss.gouv.qc.ca
514-890-8000, ext. 30331
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