Molecular pathology

The CHUM Research Centre's molecular pathology laboratory offers several services that aim to support researchers with the histological and pathological aspects of their projects.

The laboratory offers a range of flexible services and specialized equipment making it possible to meet a variety of needs, including:

  • H&E staining
  • Paraffin block or fresh tissue section
  • Construction of tissue microarrays (TMAs)
  • DNA and RNA extraction as well as sample quality control
  • Immunohistochemical or immunofluorescent staining
  • Slide digitization
  • Image analyses

Our services are available to researchers from universities, hospitals and for projects in partnership with the industry.
 

EQUIPMENTS AND SERVICES

Equipments

  • Microtome (Leica Microsystems, RM2125)
  • Microscope (Olympus Optical, BX31TF)
  • Automated IHC slide staining system (Ventana/Roche, Benchmark)
  • Cryostat ( Leica Microsystems et CM1950110111)
  • Histology manual tissue arrayer (TMA) (Beecher, 4508-DM et Pathology Devices, TM Arrayer)
  • Microscope slide scanner (Leica, Aperio Versa 200)
  • H&E slide stainer (Shandon, Varistain XY)
  • Qiacube (Qiagen)
  • Tape Station (Agilent, 4150)

Services

  • Paraffin embedding of tissues in blocks
  • Microtome sectioning
  • DNA and RNA extraction as well as sample quality control
  • Microscopy
  • Cryostat sectioning
  • Automated IHC slide staining system
  • H&E coloration
  • Tissue microarray (TMA)
  • Cell pellet preparation
  • Sampling of tissues in paraffin blocks (punch)
  • Retrieval of blocks and slides
  • Automated image analysis
  • Investigative histopathology (Feryel Azzi ;
    feryel.azzi.chum@ssss.gouv.qc.ca)

Equipment and services guide
 

SERVICE REQUEST

For any service or submission request, please fill out the electronic form.

Supervisor: Liliane Meunier, M.Sc. et Véronique Barrès, M.Sc.
Specialists: Isabelle Clément, M.Sc. et Feryel Azzi, Investigative histopathology - PHQ ITMT
Scientific advisor: Anne-Marie Mes-Masson, Ph.D.

pathologie.moleculaire.cr.chum@ssss.gouv.qc.ca
514-890-8000, ext. 30331
 

FEES

+ Fees 2022-2023
 

PUBLICATIONS

+ Publication list

 

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